SEC_Fit

Simple program to fit gaussians to analytical SEC data and estimate molecular weight from known calibrant elution volumes.

Example SEC Trace

Here is a typical SEC trace for a protein sample:

After fitting Gaussians to the peaks:

Usage

Run the program on a CSV from an Akta system:

python main.py trace.csv

The program will detect injection volumes in the CSV and ask you to pick which one to use for fitting.